Different Binding Property of Verotoxin-1 and Verotoxin-2 Against Their Glycolipid Receptor, Globotriaosylceramide.

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Toxicity and immunogenicity of a verotoxin 1 mutant with reduced globotriaosylceramide receptor binding in rabbits.

The verotoxins (VT1 and VT2), produced by strains of enterohemorrhagic Escherichia coli, have been implicated in the pathogenesis of hemorrhagic colitis and the hemolytic uremic syndrome. To better understand the role of globotriaosylceramide (Gb3) receptor binding by the verotoxins in disease production, we examined the clinicopathologic effects of an intravenously (i.v.) administered verotoxi...

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Verotoxin-1 Treatment or Manipulation of its Receptor Globotriaosylceramide (Gb3) for Reversal of Multidrug Resistance to Cancer Chemotherapy

A major problem with anti-cancer drug treatment is the development of acquired multidrug resistance (MDR) of the tumor cells. Verotoxin-1 (VT-1) exerts its cytotoxicity by targeting the globotriaosylceramide membrane receptor (Gb3), a glycolipid associated with multidrug resistance. Gb3 is overexpressed in many human tumors and tumor cell lines with inherent or acquired MDR. Gb3 is co-expressed...

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NMR studies on the dynamics and structure of Verotoxin-1 B subunits binding to its functional plasma membrane receptor, Globotriaosylceramide, Gb3

Introduction Verotoxin (VT) was first reported by Konowalchuk et al. in 1977. Although VT-producing E. coli strains belong to several different serotypes, 0157:H7 is the dominant serotype isolated from patients suffering from food poisoning. The infection frequently develops into hemorrhagic colitis. Furthermore, about 10% of these cases develop into the haemolytic uremic syndrome, which is the...

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Haemorrhagic colitis: detection of verotoxin

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Detection of verotoxin in stool specimens.

A total of 132 fecal specimens containing verotoxin (VT) were subjected to counter-current immunoelectrophoresis (CIE). Of these, 113 (85.6%) were found to be positive by CIE. Another 71 stool specimens containing E. coli serogroup O157 but with flagellar antigens other than H7 were tested for verotoxin by CIE. These stool specimens were negative for VT on Vero cell monolayers. Of these 71 stoo...

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ژورنال

عنوان ژورنال: The Tohoku Journal of Experimental Medicine

سال: 2001

ISSN: 0040-8727,1349-3329

DOI: 10.1620/tjem.195.237